[BIGBOOMER]

ever hear of something called cochline dip ? if so where to get it?

[TheBagman]

Never heard of it. :shrug:

What's it?

[taco]

new to me?

[BIGBOOMER]

it was used back in the 60s for MJ grows not in the soil but on the bud
hard to explain if you heard of it you would know what it is

[elroypornlab]

O.K

[TacoSusio]

This one of those peeonyourplant remedies?

[elroypornlab]

^LOL

[BIGBOOMER]

no not a pee on your plants LOL
but some old school tek

[BIGBOOMER]

thank you for your time but i found what it is
found this post from another site


Colchicine:

The chemical name for colchicine or Acetyltrimethylcolchicinic acid is: (S)-N-(5,6,7,9-tetrahydro-1,2,3,10-tetramethoxy- 9-oxobenzo (a) heptalen-7-yl) acetamide (molecular weight 399.43).

Molecular Formula: C22H25NO6



Colchicine consists of pale yellow Scales or powder; it darkens on exposure to light. Colchicine is an alkaloid soluble in water, freely soluble in alcohol and in chloroform, and slightly soluble in ether. It is named after Colchicum, a plant containing colchicine.



Optimum storage temperature: -15 °C to -25 °C, in dark coloured bottles (breakdown in light).
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Working method

Colchicine is an antimitotic agent, that blocks or suppresses cell division by inhibiting mitosis, the division of a cell's nucleus. Specifically, it inhibits the development of spindles as the nuclei are dividing. (Spindles are formed by the polymerization of tubulin from a pool of subunits during a discrete phase of the cell cycle and then depolymerized during another phase.) Normally, the cell would use its spindle fibers to line up its chromosomes, make a copy of them, and divide into two new cells with each daughter cell having a single set of chromosomes. With colchicine present, the spindle fibers don't form, and so the cell can't move its chromosomes around. The cell may end up copying some or all of the chromosomes anyway, but can't parcel them out into new cells, and so it never divides.

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Use

Colchicine can be used to induce polyploidy. It induces sometimes also other mutations like chlorophyll mutations, but the frequency is very low.

It can solve an important problem in Fuchsia breeding: Most Fuchsia species are diploid or tetraploid (tetraploid species are for instance F. magellanica, F. magdalenae and F. triphylla). A crossing between a diploid and a tetraploid results often in a triploid, which is mostly sterile and can't be used for further breeding. Doubling the chromosome number from the diploid parent or the triploid can solve that problem.
A triploid is sterile because the process of meiosis [cell division for reproduction] requires the pairing of similar chromosomes and because there is no mechanism allowing for the alignment of three similar chromosomes, triploid plants are not able to produce fertile reproductive cells. They are therefore sterile and unusable as parents. For instance: triploid daylilies do not produce reproductive cells of 16-1/2 chromosomes, which can combine to form triploid children of 33 chromosomes per cell, in the way that diploid parents produce reproductive cells of 11 chromosomes or tetraploid parents produce reproductive cells of 22 chromosomes which then combine to produce diploid children with 22 chromosomes or tetraploid children with 44 chromosomes. However, this is not always true. Experiences with Hemerocallis (daylily) 'Europa' for instance seems to indicate that viable pollen is produced about 15% of the time, and viable ovules about 1% of the time, for crossing with diploids. (A.B. Stout made 7,135 crosses onto the known triploid Hemerocallis fulva 'Europa' using diploid pollen and obtained 70 seeds (presumably all diploids)).

An other advantage of Polyploid plants: all plantparts are bigger (flowers, leaves). A lot of big double Fuchsia's are polyploid.

A special problem of colchicine-induced ploidy, in particular in vegetatively propagated crops, is the chimerism caused by the simultanous present of tissue of different ploidy levels in one plant or plant part.

Colchicine is mostly applied feshly prepared in aqueous solutions. The range of concentrations of colchicine applied varies considerably, for instance from 0.006-3 %. I think a concentration of about 0.05% is the most common used concentration.

Colchicine can also be applied in a lanolin paste or as as a solution, for instance, on a cotton dot, placed in a leaf axil.

Colchicine works in dividing cells and those can be found in buds, tops of shoots and seeds. If you want to use it on Fuchsia, I think the best way is to treat cuttings or seeds in a 0.05% solution for 3-5 days, or perhaps inject a solution. I have no literature about treating Fuchsia with colchicine, so it is a matter of trial and error.
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[TacoSusio]

Ok, but why use it?

[taco]

Originally Posted by TacoSusio

Ok, but why use it?

I think this is what hes geeting at.

Originally Posted by BIGBOOMER

An other advantage of Polyploid plants: all plantparts are bigger (flowers, leaves). A lot of big double Fuchsia's are polyploid.

[TacoSusio]

ah, thanks taco!

[taco]

Originally Posted by TacoSusio

ah, thanks taco!

I do pay attention every now and then.